Biotechnology
M. Ahmadi-Roshan; G. Karimzadeh; S. Rashidi monfared
Abstract
Satureja bachtiarica Bunge is one of the endemic Satureja species in Iran, which has strong antioxidant and antimicrobial properties as well as economic and medicinal vlaue due to the presence of compounds like carvacrol and thymol in the essential oil and rosmarinic acid and other phenolic acids in ...
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Satureja bachtiarica Bunge is one of the endemic Satureja species in Iran, which has strong antioxidant and antimicrobial properties as well as economic and medicinal vlaue due to the presence of compounds like carvacrol and thymol in the essential oil and rosmarinic acid and other phenolic acids in the extract. In this study, 10 shooting and 8 rooting treatments were used in MS medium to produce clones of this species using micropropagation method. The shooting treatments were a combination of cytokinins (Kin, 2ip, and TDZ) and auxins (IBA and BA) with different concentrations. The rooting treatments were also a combination of different concentrations of auxins (IBA and NAA). The best shooting treatment [IBA (0.01 mg l-1), TDZ (0.05 mg l-1), and 2ip (0.3 mg l-1)] was recognized by examining traits such as number and length of main and secondary shoots, number of leaves, number of lateral active buds, and leaf colour. Number of main and lateral roots were also criteria for the best rooting treatment [IBA (0.5 mg l-1) and NAA (0.5 mg l-1)] selection. Since S. bachtiarica is an allogamous plant and has a high diversity for different traits like metabolites, practical purpose of the present study was mass production of identical individuals for use in breeding programs. This purpose was done by presenting the micropropagation protocol of this valuable plant.
S.H. Madani; B. Hosseini; Gh. Karimzadeh; A. Rahimi
Abstract
Iranian poppy (Papaver bracteatum Lindl.) is one of the medicinal plants belonging to the Papaveraceae family, which is widely used in pharmaceutical industries due to the presence of benzyl isoquinoline alkaloids. The polyploidy induction is one of the most interesting issues in the breeding and biotechnology ...
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Iranian poppy (Papaver bracteatum Lindl.) is one of the medicinal plants belonging to the Papaveraceae family, which is widely used in pharmaceutical industries due to the presence of benzyl isoquinoline alkaloids. The polyploidy induction is one of the most interesting issues in the breeding and biotechnology of medicinal plants. In this study, colchicine treatment was carried out in five concentrations (0, 0.05, 0.1, 0.2 and 0.5) and three duration times (24, 48, 72 hours) with three replications as a factorial in a completely randomized design under greenhouse conditions, which aimed at studying morphological and phytochemical changes in polyploid plants and comparing them with diploid ones. The Microscopic, morphological and chromosomal counts were used to determine ploidy level of plants. The results showed that the concentration of 0.2% of colchicine for 48 hours was the most suitable treatment for polyploidy induction. Polyploidy caused significant changes in the increasing phytochemicals amount such as phenol, flavonoids and total antioxidants (DPPH) and decreasing the stomatal density index in comparison with the diploid plants. The chromosomes number of the diploid and tetraploid plants was obtained 14 (2n=2x=14) and 28 (2n=4x=28), respectively. Therefore, it can be concluded that the increase in ploidy level increases the phytochemical and antioxidant compounds in Iranian poppy herb.
